|
| Status |
Public on Jun 10, 2012 |
| Title |
Comparing two transcriptome technologies - sequencing match microarrays [Array] |
| Organism |
Streptococcus thermophilus |
| Experiment type |
Expression profiling by array
|
| Summary |
Next-generation sequencing has emerged as a promising platform for whole genome transcriptome profiling. The microarray technology has in contrast a long track record, but may suffer from issues related to background fluorescence and non-specific hybridization. The two technologies have been compared for eukaryotic transcriptomes in various studies. However, for prokaryotes information on comparative performance of the two technologies is sparse. We thus compared the two technologies for Streptococcus thermophilus. With just single experiments there was a strong correlation between the technologies.
|
| |
|
| Overall design |
We compared our in house spotted DNA microarray platform with the same RNA samples sequenced externally. The aim was to, without replicate arrays or sequencing, to determine if the two technologies correlate well.
|
| |
|
| Contributor(s) |
Rasmussen TB, Dantoft SH, Pedersen MB |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Dec 15, 2011 |
| Last update date |
Jun 11, 2012 |
| Contact name |
Martin Bastian Pedersen |
| E-mail(s) |
martinbastianpedersen@gmail.com
|
| Organization name |
Sacco Srl
|
| Department |
R&D
|
| Street address |
Via Manzoni 29
|
| City |
Cadorago |
| State/province |
CO |
| ZIP/Postal code |
IT-22071 |
| Country |
Italy |
| |
|
| Platforms (1) |
| GPL15014 |
Chr. Hansen A/S_Streptococcus thermophilus_2304_v2.0 |
|
| Samples (1) |
| GSM849537 |
CHCC5086_versus_CHCC5376_Milk_pH5.7 |
|
| This SubSeries is part of SuperSeries: |
| GSE34478 |
Comparing two transcriptome technologies - sequencing match microarrays |
|
| Relations |
| BioProject |
PRJNA156347 |
