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| Status |
Public on Apr 06, 2017 |
| Title |
Role of CRP K100 positive charge on Escherichia coli global transcriptome |
| Organism |
Escherichia coli |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
We hypothesized that the positive charge of CRP K100 is important for transcription of Class II CRP-dependent genes. These data show mutations that affect K100 charge status result in genome-wide changes in transcription of both CRP-dependent and -independent genes.
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| Overall design |
Total RNA obtained from E.coli strains overexpressing WT CRP or a K100 variant (K100Q or K100R) grown in tryptone broth buffered to pH 7 (TB7) supplemented with 22 mM glucose. A strain overexpressing an AR1-/AR2- variant was used as a negative control.
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| |
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| Contributor(s) |
Kuffel GR |
| Citation missing |
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| Submission date |
Apr 05, 2017 |
| Last update date |
May 15, 2019 |
| Contact name |
Alan J Wolfe |
| E-mail(s) |
awolfe@luc.edu
|
| Organization name |
Loyola University - Chicago
|
| Department |
Microbiology and Immunology
|
| Street address |
2160 S. 1st Ave, Bldg 105 Rm 224
|
| City |
Maywood |
| State/province |
Illinois |
| ZIP/Postal code |
60153 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL16085 |
Illumina MiSeq (Escherichia coli) |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA381697 |
| SRA |
SRP103070 |
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