|
| Status |
Public on Jun 22, 2017 |
| Title |
Lcr35® restituo capsule_2 |
| Sample type |
RNA |
| |
|
| Source name |
Native strain_capsule
|
| Organism |
Lacticaseibacillus rhamnosus |
| Characteristics |
strain: Lcr35®
manufacturing process: capsule
|
| Growth protocol |
Lactobacillus rhamnosus Lcr35 native strain and formulations were grown overnight at 37°C in De Man Rogosa Sharpe (MRS) medium (BIORAD, Mitry Mory, France). 59(6):2075).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using the RNA L kit (Macherey Nagel, Duren, Germany) and DNA was removed using DNase digestion with the RNase-Free Dnase kit (Ambion). RNA quality and concentration was determined by analysis with an Agilent 2100 bioanalyzer.
|
| Label |
Cy3
|
| Label protocol |
Labeling was performed by NimbleGen Systems Inc., Madison, WI USA, following their standard operating protocol. See www.nimblegen.com.
|
| |
|
| Hybridization protocol |
Hybridization was performed by NimbleGen Systems Inc., Madison, WI, USA following their standard operating protocol. See www.nimblegen.com.
|
| Scan protocol |
Scanning was performed by NimbleGen Systems Inc., Madison, WI USA, following their standard operating protocol. See www.nimblegen.com.
|
| Data processing |
The raw data (.pair file) was subjected to RMA (Robust Multi-Array Analysis; Irizarry et al. Biostatistics 4(2):249), quantile normalization (Bolstad et al. Bioinformatics 19(2):185), and background correction as implemented in the NimbleScan software package, version 2.4.27 (Roche NimbleGen, Inc.).
|
| |
|
| Submission date |
Jun 25, 2014 |
| Last update date |
Jun 22, 2017 |
| Contact name |
Stephanie Bornes |
| E-mail(s) |
stephanie.bornes@udamail.fr
|
| Organization name |
Universite d'Auvergne
|
| Department |
IUT genie biologique
|
| Street address |
100 rue de l'egalite
|
| City |
Aurillac |
| ZIP/Postal code |
15013 |
| Country |
France |
| |
|
| Platform ID |
GPL18863 |
| Series (1) |
| GSE58820 |
Influence of manufacturing processes on cell surface properties of probiotic strain Lactobacillus rhamnosus Lcr35® |
|
