|
| Status |
Public on Jan 29, 2016 |
| Title |
GLY+DMSO+PEG6 |
| Sample type |
genomic |
| |
|
| Source name |
human fecal sample
|
| Organism |
uncultured bacterium |
| Characteristics |
gender: Female
age (years): 83
|
| Treatment protocol |
Total DNA extracted using Qiagen Stool kit
|
| Growth protocol |
Fecal microbiota was grown in an anaerobic way in Macfarlane media.
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
16S bacterial genes amplified using universal primers, next labelled with Cy3 or Cy5 dyes and hybridized to the DNA microarray probes as stated before (Tottey et al, 2013).
|
| Label |
cy5
|
| Label protocol |
As published by Tottey et al, 2013
|
| |
|
| Hybridization protocol |
As published by Tottey et al, 2013
|
| Scan protocol |
Agilent Technologies, Palo Alto, CA
|
| Description |
Glycerol+DMSO+PEG preserved sample 6 months
raw data available in cy5 channel of file:
US91603675_256599410024_S01_CGH_105_Dec08_Classique6.txt
|
| Data processing |
PhylInterpret software
|
| |
|
| Submission date |
Jan 28, 2016 |
| Last update date |
Jan 29, 2016 |
| Contact name |
Nadia Gaci |
| E-mail(s) |
luckypgi@gmail.com
|
| Phone |
+33782948558
|
| Organization name |
University d Auvergne
|
| Department |
Pharmacy
|
| Lab |
EA-CIDAM 4678
|
| Street address |
28, Place H. Dunant - B.P. 38
|
| City |
Clermont Ferrand |
| State/province |
Auvergne |
| ZIP/Postal code |
63001 |
| Country |
France |
| |
|
| Platform ID |
GPL16731 |
| Series (1) |
| GSE77327 |
Human gut microbiota preservation for functional studies: in-vitro comparison of common cryoprotective agents |
|
