|
| Status |
Public on Aug 16, 2013 |
| Title |
R0052 Genomic DNA |
| Sample type |
genomic |
| |
|
| Source name |
Genomic DNA isolated from overnight culture
|
| Organism |
Lactobacillus helveticus |
| Characteristics |
strain: R0052
|
| Treatment protocol |
No treatments were applied to cells prior to DNA isolation
|
| Growth protocol |
All cultures were grown in MRS broth at 37C for 24 hours before Genomic DNA was isolated
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Genomic DNA was isolated using the Masterpure Gram Positive DNA Purification Kit from Epicentre Biotechnologies (Cat. No. #MGP04100
|
| Label |
biotin
|
| Label protocol |
Biotinylated DNA was prepared according to the standard Affymetrix protocol (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Done according to the standard Affymetrix protocol (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| Scan protocol |
Done according to the standard Affymetrix protocol (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| Description |
Genomic DNA
|
| Data processing |
The data were analyzed with R/Bioconductor using RMA as the normilization method.
|
| |
|
| Submission date |
Aug 16, 2010 |
| Last update date |
Aug 16, 2013 |
| Contact name |
Jeff Broadbent |
| E-mail(s) |
broadbent.jeff@gmail.com, rlthompson28@hotmail.com
|
| Phone |
435-797-2146
|
| Fax |
435-797-2379
|
| Organization name |
Utah State University
|
| Department |
NFS
|
| Street address |
750 N 120 E
|
| City |
Logan |
| State/province |
UT |
| ZIP/Postal code |
84322 |
| Country |
USA |
| |
|
| Platform ID |
GPL9271 |
| Series (1) |
| GSE23660 |
Genome heterogeneity in Lactobacillus helveticus: Rethinking a “niche specialist”* |
|
