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| Status |
Public on Aug 16, 2013 |
| Title |
Genome heterogeneity in Lactobacillus helveticus: Rethinking a “niche specialist”* |
| Platform organisms |
Lactobacillus helveticus; Lacticaseibacillus paracasei ATCC 334 |
| Sample organism |
Lactobacillus helveticus |
| Experiment type |
Genome variation profiling by array
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| Summary |
Lactobacillus helveticus is a rod-shaped lactic acid bacterium that is widely used in the manufacture of fermented dairy foods and for production of bioactive peptides from milk proteins. Although L. helveticus is commonly associated with milk environments, phylogenetic studies show it is closely related to an intestinal species, Lactobacillus acidophilus, which has been shown to impart probiotic health benefits to humans. This relationship has fueled a prevailing hypothesis that L. helveticus is a highly specialized derivative of L. acidophilus which has adapted to acidified whey. However, L. helveticus has also been sporadically recovered from non-dairy environments, which argues the species may not be as highly specialized as is widely believed. This study employed genome sequence analysis and comparative genome hybridizations to investigate genomic diversity among L. helveticus strains collected from cheese, whey, and whiskey malt, as well as commercial cultures used in manufacture of cheese or bioactive dairy foods. Results revealed considerable variability in gene content between some L. helveticus strains, and indicated the species should not be viewed as a strict dairy-niche specialist. In addition, comparative genomic analyses provided new insight on several industrially and ecologically important attributes of L. helveticus that may facilitate commercial strain selection.
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| Overall design |
42 samples were hybridized to the microarray chip, which contains probe sequences from L. helveticus CNRZ32. CNRZ32 was also hybridized and used as the reference sample. Data from the microarray was statistically analyzed using the R software. Samples were compared to the reference (CNRZ32) to investigate genome diversity amoung L. helveticus strains,
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| Contributor(s) |
Broadbent JR, Cai H, Hughes J, Larsen RL, Welker DL, de Carvalho V, Tompkins T, Barreau G, Swiatek M, Steele JL, Ardö Y, Vogensen F, De Lorentiis A, Gatti M, Neviani E |
| Citation missing |
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| Submission date |
Aug 16, 2010 |
| Last update date |
Aug 17, 2013 |
| Contact name |
Jeff Broadbent |
| E-mail(s) |
broadbent.jeff@gmail.com, rlthompson28@hotmail.com
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| Phone |
435-797-2146
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| Fax |
435-797-2379
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| Organization name |
Utah State University
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| Department |
NFS
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| Street address |
750 N 120 E
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| City |
Logan |
| State/province |
UT |
| ZIP/Postal code |
84322 |
| Country |
USA |
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| Platforms (1) |
| GPL9271 |
Lactobacillus casei ATCC 334 5.1K |
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| Samples (42)
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| Relations |
| BioProject |
PRJNA130859 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE23660_RAW.tar |
18.4 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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