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Sample GSM580457 Query DataSets for GSM580457
Status Public on Aug 16, 2013
Title CNRZ32-1992 Genomic DNA
Sample type genomic
 
Source name Genomic DNA isolated from overnight culture
Organism Lactobacillus helveticus
Characteristics strain: CNRZ32-1992
Treatment protocol No treatments were applied to cells prior to DNA isolation
Growth protocol All cultures were grown in MRS broth at 37C for 24 hours before Genomic DNA was isolated
Extracted molecule genomic DNA
Extraction protocol Genomic DNA was isolated using the Masterpure Gram Positive DNA Purification Kit from Epicentre Biotechnologies (Cat. No. #MGP04100
Label biotin
Label protocol Biotinylated DNA was prepared according to the standard Affymetrix protocol (Expression Analysis Technical Manual, 2001, Affymetrix).
 
Hybridization protocol Done according to the standard Affymetrix protocol (Expression Analysis Technical Manual, 2001, Affymetrix).
Scan protocol Done according to the standard Affymetrix protocol (Expression Analysis Technical Manual, 2001, Affymetrix).
Description Genomic DNA
Data processing The data were analyzed with R/Bioconductor using RMA as the normilization method.
 
Submission date Aug 16, 2010
Last update date Aug 16, 2013
Contact name Jeff Broadbent
E-mail(s) broadbent.jeff@gmail.com, rlthompson28@hotmail.com
Phone 435-797-2146
Fax 435-797-2379
Organization name Utah State University
Department NFS
Street address 750 N 120 E
City Logan
State/province UT
ZIP/Postal code 84322
Country USA
 
Platform ID GPL9271
Series (1)
GSE23660 Genome heterogeneity in Lactobacillus helveticus: Rethinking a “niche specialist”*

Data table header descriptions
ID_REF
VALUE log-2 signal intensity average value

Data table
ID_REF VALUE
AFFX-Athal-25SrRNA_s_at 2.513222
AFFX-Athal-5SrRNA_at 2.216021
AFFX-Athal-Actin_3_f_at 2.133004
AFFX-Athal-Actin_5_r_at 1.906523
AFFX-Athal-Actin_M_at 2.45145
AFFX-Athal-GAPDH_3_s_at 1.96031
AFFX-Athal-GAPDH_5_s_at 1.823076
AFFX-Athal-GAPDH_M_s_at 1.943481
AFFX-Athal-Ubq_3_f_at 2.088262
AFFX-Athal-Ubq_5_f_at 1.971067
AFFX-Athal-Ubq_M_f_at 1.727102
AFFX-BioB-3_at 1.189419
AFFX-BioB-5_at 1.491224
AFFX-BioB-M_at 1.333955
AFFX-BioC-3_at 1.179975
AFFX-BioC-5_at 1.272209
AFFX-BioDn-3_at 1.35481
AFFX-BioDn-5_at 1.338018
AFFX-CreX-3_at 1.443812
AFFX-CreX-5_at 1.445178

Total number of rows: 5148

Table truncated, full table size 132 Kbytes.




Supplementary file Size Download File type/resource
GSM580457.CEL.gz 408.1 Kb (ftp)(http) CEL
Processed data included within Sample table

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