BioProject

Metabolic diseases such as type 2 diabetes (T2D) and T2D-related comorbidities (eg. More...

Metabolic diseases such as type 2 diabetes (T2D) and T2D-related comorbidities (eg. obesity, metabolic syndrome, pre-diabetes and impaired glucose tolerance) are major public health issues worldwide. Recent experiments in animal and human studies have generated growing evidence for the association of gut microbiome and development of these diseases. Using next generation sequencing, large amounts of metagenomic data have been generated from T2D and control subjects. However, the disease-associated prokaryotic repertoire of the human gut microbiota is still undefined. Here we identified 389 uncultured novel prokaryotic species, including 388 bacteria and 1 archaea, by reconstructing 95871 metagenome-assembled genomes (MAGs) from 5814 disease-associated human gut microbiomes and de-replicating the MAGs against a comprehensive human gut library (ERP108418). Amongst these uncultured genomes, 302 are novel species and 87 are related to known species but have never been found in the human gut before. Our work expands the knowledge of the diversity of uncultured human gut bacteria associated with metabolic diseases, and can be used as a valuable reference library to help improve the species detection in future disease-associated metagenomics studies. Less...