GEO DataSets

(Submitter supplied) In this work, high-throughput RNA sequencing was used to assess transcriptomic alteration in Burkholderia cenocepacia K56-2 in the early stages of interaction with host-cell surface. Giant plasma membrane vesicles (GPMVs) from 16HBE14o- bronchial epithelial cell line were produced and used as a cell-like alternative. RNAseq was performed and the expression of the adherent (GPMV-attached) and non-adherent (planktonic control) bacteria were compared. more...

Organism:

Burkholderia cenocepacia

Type:

Expression profiling by high throughput sequencing

Platform:

GPL28995

6 Samples

Download data: TXT

(Submitter supplied) Purpose - To establish the response of B. cenocepacia K56-2 to growth in copper, and to characterise the BCAM0442/3 copper-sensing TCS. Methods - RNA-seq of the response WT and Δbcam0442/3 B. cenocepacia K56-2 to mid-log phase growth in LB with or without 1 mM CuCl2 was performed. Results - Characterisation of the overall response of B. cenocepacia K56-2 to copper, as well as establishing that BCAM0442/3 regulates the CopABCDE system in B. more...

Organism:

Burkholderia cenocepacia

Type:

Expression profiling by high throughput sequencing

Platform:

GPL29736

12 Samples

Download data: CSV

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Burkholderia cenocepacia

Type:

Genome binding/occupancy profiling by high throughput sequencing; Expression profiling by high throughput sequencing

Platform:

GPL19185

11 Samples

Download data

(Submitter supplied) BACKGROUND: We identified that a putative CzcRS-like two-component system (TCS) in Burkholderia cenocepacia K56-2 is required for heavy metal resistance and virulence. To investigate this TCS further, we applied RNA-seq to determine the transcriptional response of wildtype and CzcRS-deficient B. cenocepacia to subinhibitory concetrations of zinc. METHODS. Wildtype B. cenocepacia K56-2 was cultured in LB broth with and without 1.5 mM zinc chloride. more...

Organism:

Burkholderia cenocepacia

Type:

Expression profiling by high throughput sequencing

Platform:

GPL19185

9 Samples

Download data: TAB, TXT

(Submitter supplied) BACKGROUND: We identified that a putative CzcRS-like two-component system (TCS) in Burkholderia cenocepacia K56-2 is required for heavy metal resistance and virulence. In an attempt to identify genes directly regulated by the CzcR response regulator, we performed ChIP-seq analysis to identify genomic regions bound by CzcR. METHODS. Sequence encoding a FLAG octapeptide (Sigma-Aldrich) was introduced to the 3’ end of the czcR gene at its native chromosomal position in wild-type B. more...

Organism:

Burkholderia cenocepacia

Type:

Genome binding/occupancy profiling by high throughput sequencing

Platform:

GPL19185

2 Samples

Download data: TXT

(Submitter supplied) Individual normalized intensity of Burkholderia cenocepacia and Burkholderia seminalis hybridized in a B. cenocepacia array

Organism:

Burkholderia cenocepacia; Burkholderia seminalis

Type:

Genome variation profiling by array

Platforms:

GPL18793 GPL18776

429 Samples

Download data: TXT

(Submitter supplied) Hfq proteins are RNA chaperones that play a critical role in post-transcription regulation of gene expression. Bacteria of the Burkholderia cepacia complex harbor two distinct and functional Hfq proteins, the Hfq and Hfq2. We have previously performed the functional analysis of Hfq and Hfq2 in the pathogen Burkholderia cenocepacia J2315. In order to examine the impacts of each RNA chaperone on the global transcriptome of B. more...

Organism:

Burkholderia cenocepacia J2315; Burkholderia cenocepacia; Burkholderia multivorans ATCC 17616

Type:

Expression profiling by array

Platform:

GPL13356

9 Samples

Download data: CEL, TXT

(Submitter supplied) comparative genomic hybridization between Burkholderia cepacia sequenced strains to determine what are the limits to strain relativity in order to still provide accurate estimations of gene content

Organism:

Burkholderia cenocepacia J2315; Paraburkholderia xenovorans LB400; Burkholderia ambifaria AMMD; Burkholderia cenocepacia; Burkholderia vietnamiensis G4; Burkholderia cenocepacia HI2424; Burkholderia orbicola

Type:

Genome variation profiling by array

Platform:

GPL9990

12 Samples

Download data: TXT

(Submitter supplied) [1] Transcription profiling of one Burkholderia cenocepacia clinical isolate, J2315, versus a soil isolate, HI2424, in conditions mimicking CF sputum [2] Transcription profiling of Burkholderia cenocepacia isolates J2315 and HI2424 in media mimicking CF sputum or the soil environment

Organism:

Burkholderia cenocepacia

Type:

Expression profiling by array

Platform:

GPL8475

8 Samples

Download data: TXT

(Submitter supplied) Transcriptional profiling of RNA-seq data from two Burkholderia species grown under conditions mimicking the cystic fibrosis lung and the soil environment

Organism:

Burkholderia cenocepacia

Type:

Expression profiling by high throughput sequencing

Platform:

GPL9702

8 Samples

Download data: TXT, XLS

(Submitter supplied) http://www.mycroarray.com/technology/index.html

Organism:

Burkholderia cenocepacia

1 Series

417 Samples

Download data: GAL

(Submitter supplied) http://www.mycroarray.com/technology/index.html

Organism:

Burkholderia cenocepacia

1 Series

12 Samples

Download data: GAL

(Submitter supplied) see the manufacturer's website

Organism:

Burkholderia cenocepacia

1 Series

12 Samples

Download data: GAL

(Submitter supplied) Agilent

Organism:

Burkholderia cenocepacia

1 Series

8 Samples

Download data