GEO DataSets

(Submitter supplied) A prototype oligonucleotide microarray was designed to detect and identify viable bacterial species with the potential to grow of common beer spoilage microorganisms from the genera Lactobacillus, Megasphaera, Pediococcus and Pectinatus. Probes targeted the intergenic spacer regions (ISR) between 16S and 23S rRNA, which were amplified in a combination of reverse transcriptase (RT) and polymerase chain reaction (PCR) prior to hybridization. more...

Organism:

Pectinatus frisingensis; Levilactobacillus brevis; Pediococcus damnosus; Pectinatus cerevisiiphilus; Lacticaseibacillus casei; Megasphaera cerevisiae; Loigolactobacillus coryniformis; Schleiferilactobacillus perolens; Pediococcus inopinatus

Type:

Expression profiling by array

Platforms:

GPL5157 GPL5158

12 Samples

Download data: GPR

(Submitter supplied) DNA sequences for the 3163 C. thermocellum ATCC 27405 predicted protein-encoding genes were obtained from The Joint Genome Institute using sequence assembled in November 2003. Oligonucleotide probes that represented the whole genome of C. thermocellum were designed using the CommOligo software and were commercially synthesized without modification (MWG Biotech, High Point, NC) in 96-well plates. The concentration of the probes was adjusted to 100 pmol/JlL, transferred to 384-well printing plates in a final concentration of 50% dimethyl sulfoxide using a BioMek FX liquid handling robot (Beckman-Coulter, Fullerton, CA) and then spotted onto UltraGAPS glass slides (Corning Life Sciences, Corning, NY) using a BioRobotics Microgrid II microarrayer (Genomic Solutions, Ann Arbor, MI) in a dust-free clean room maintained at 21°C and 50% relative humidity. more...

Organism:

Acetivibrio thermocellus ATCC 27405

1 Series

39 Samples

Download data: TXT

(Submitter supplied) BACKGROUND: Microarray comparative genomic hybridization (CGH) is currently one of the most powerful techniques to measure DNA copy number in large genomes. In humans, microarray CGH is widely used to assess copy number variants in healthy individuals and copy number aberrations associated with various diseases, syndromes and disease susceptibility. In model organisms such as Caenorhabditis elegans (C. more...

Organism:

Caenorhabditis elegans; Homo sapiens

Type:

Genome variation profiling by genome tiling array

Platforms:

GPL7085 GPL7084

3 Samples

Download data: PAIR

(Submitter supplied) Data from the VLA lyssavirus genotyping microarray. The array platform for this data is GEO accession GPL8066, and consists of 624 oligos representing two viral families. The data set itself consists of 14 arrays, 7 hybridised with RNA from mice brains infected with 7 genotypes of lyssaviruses, 1 hybridised with RNA from normal mouse brain, and 6 hybridised with RNA from coded samples consisting of infected mouse brains or control mouse brains. more...

Organism:

Lyssavirus rabies; Lyssavirus lagos; European bat lyssavirus 1; Lyssavirus australis; Lyssavirus duvenhage; Viruses; Lyssavirus mokola; unidentified; European bat lyssavirus 2

Type:

Other

Platform:

GPL8066

14 Samples

Download data: GPR

(Submitter supplied) Microarrays have become a powerful tool for DNA-based molecular diagnostics and identification of pathogens. However, most of them target a limited range of organisms and are generally based on only one or very few genes for organism identification. Although such microarrays are proven tools for species identification, they suffer from the fact that identification is only possible for organisms for which probes were specifically pre-developed. more...

Organism:

Micrococcus luteus; Escherichia coli K-12; synthetic construct; Pantoea stewartii; Salmonella enterica subsp. enterica serovar Typhimurium; Salmonella enterica subsp. enterica serovar Typhimurium str. LT2; Pantoea agglomerans; Escherichia coli B

Type:

Genome variation profiling by array

Platform:

GPL8295

23 Samples

Download data: PAIR

(Submitter supplied) Semiconductor based array with in situ synthesized oligonucleotides that can be used with electrochemical detection. Protocol: The custom oligonucleotide microarray is synthesized on a semiconductor using an electrochemical synthesis process. Each oligonucleotide probe (spot) is synthesized on a platinum electrode that is independently controlled by the synthesizer’s computer. Synthesis is based on established phosphoramidite chemistry and occurs at thousands of sites simultaneously according to a computer algorithm that activates only specified electrodes. more...

Organism:

Influenza A virus

1 Series

26 Samples

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